FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_395-14-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_395-14-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14234930
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG10653477.484033992439724TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172490.12117376060156249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA1439100.058.13527359
GCTTGAA1454500.057.9923860
AAAAAAG1461200.057.25183569
CTTGAAA1518200.055.7114161
CTGCTTG1528100.054.69645758
GAAAAAA1603400.054.0143164
TGAAAAA1607050.053.63186363
TTGAAAA1582300.053.57679762
CCGTCTT1611650.052.76138351
TCTGCTT1588900.052.72630757
GCCGTCT1618150.052.48122450
CTTCTGC1614800.052.36363655
TTCTGCT1602300.052.13446456
CGTCTTC1630650.052.07045752
GTCTTCT1633050.051.6284653
TCTTCTG1658750.050.88054354
TGCCGTC1705100.049.8899749
ATGCCGT1738550.048.98365448
TATGCCG1869100.045.5973947
GTATGCC1915900.044.5305546
Produced by FastQC (version 0.11.2)