FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515576
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1760082.7013421376713276RNA PCR Primer, Index 24 (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98620.15136037090197396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA304950.046.35101360
AAAAAAG320250.044.0813769
TGCTTGA318800.043.9362559
CTGCTTG324700.043.0738258
CTTGAAA333000.042.58135661
GAAAAAA353900.041.1490764
TGAAAAA375400.038.5626163
TTGAAAA376400.037.90991662
TCTGCTT375700.037.29068457
GCCGTCT382850.037.06226350
CCGTCTT390050.036.51107451
TGCCGTC390700.036.34409349
ATGCCGT392750.036.18952648
CTTCTGC394800.035.95847755
TATGCCG397800.035.8081647
TTCTGCT394200.035.60187556
CGTCTTC403650.035.22964552
GTATGCC405950.035.1402546
CATCTCG399600.034.92220340
GTCTTCT405450.034.90333653
Produced by FastQC (version 0.11.2)