FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_131-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_131-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25268479
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3167571.2535657567675522TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT548050.045.59958650
TGCTTGA564100.044.10122759
CTGCTTG565650.043.90701758
GCTTGAA570700.043.863560
CCGTCTT578550.043.26730751
TGCCGTC611950.040.843749
ATGCCGT630350.039.68422748
CGTCTTC665450.037.8452152
TCTGCTT662000.037.76609457
CTTCTGC675150.037.2249155
AAAAAAG662900.036.9978169
GTCTTCT680750.036.99470553
CGTGTGC123950.036.531751
TTCTGCT688150.036.47627656
TATGCCG690350.036.16014547
CTTGAAA710050.035.65846361
GAAAAAA739950.034.5252964
GTATGCC730950.034.2226646
GATCTCG747900.032.50695440
TCTTCTG776850.032.50266354
Produced by FastQC (version 0.11.2)