FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25791946
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405373015.717038179282788No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3014041.1685973598114698Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG474280.1838868614256559No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA588750.062.3682022
GCACACG190150.061.82640518
AGAGCAC326550.059.97292315
ATAACTG634950.058.4979741
GCACGCG68900.058.45035618
ACTGAAG631000.058.4173974
AGCACAC210850.057.4160817
GAGCACA296000.055.80438216
GTCCCCG85600.055.03658345
GGTCCCC96600.054.8927344
TGGTCCC100500.054.086443
GTGACTG121950.053.967121
TCATACT302350.053.80813612
AGATCAT497400.053.739169
CATACTT290800.053.65179413
CACGCGT27200.053.2557719
TGACTGG131500.053.195052
CACGCGA36450.053.08390819
GATCGAC272100.052.90655544
GATCATA401000.052.76923410
Produced by FastQC (version 0.11.5)