FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300870
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC636092.764562969659303Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287551.2497446618018402No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC25620.11134918530816604Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG5100.044.60402345
CCCCGTA6100.040.735147
GGTCCCC6450.037.43865644
TCCCCGT6150.036.98950646
AGAGCAC16250.036.6138415
AAAGGGG116600.035.41643567
AAGGGGG116700.035.29612468
GACCGAC1600.035.00156410
GAGCACA15500.034.5461816
CGATCGG2350.034.3187641
TGGTCCC7300.033.55876543
CCCGTAT5950.032.35157448
CAGATCG50450.032.1959276
GTGGTCC7400.032.1580142
AGGGGGG132000.032.1336169
CGCCGTA188200.032.02239247
CCAGATC15750.031.561775
TCGCCGT189200.031.52018546
CGCCCTA7800.031.40824747
CCGTATC189350.031.38499549
Produced by FastQC (version 0.11.5)