FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805960
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC63562113.225682277838352Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG763741.589151803177721No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC215640.44869287301600513Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC134430.27971518697617126Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC95960.19966874464206943Illumina Single End PCR Primer 1 (100% over 49bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC94760.1971718449591757Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC85480.1778624874114641Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC83530.17380502542676177Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC79380.1651699140234209Illumina Single End PCR Primer 1 (98% over 50bp)
CGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC75050.156160267667646Illumina Single End PCR Primer 1 (98% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC69510.1446329141316199Illumina Single End PCR Primer 1 (98% over 50bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC65530.13635153018335566Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCCCC64630.13447885542118537Illumina Single End PCR Primer 1 (98% over 50bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG63940.13304313810352147Illumina Single End PCR Primer 1 (97% over 48bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGT54790.1140042780214567Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG15650.060.261631
GCACAAG57850.056.98954418
AGAGCAC99400.056.44218415
GAGCACA92850.056.425916
AGCACAA58400.055.37353517
GTGACTG38950.054.546651
TGGTCCC52200.054.37484443
GTCCCCG48700.053.68219445
CCCCGTA54150.053.57845347
GGTCCCC52100.053.53763644
GTGGTCC51800.053.50988842
AAGAGCA133400.052.36744314
TCCCCGT50850.052.0313346
GACTAGT27150.050.79109612
TCGCAAC11450.050.7431410
TGACTGG43200.050.2741052
AGATAGA100200.049.5358287
AGTTCGG10000.049.4335821
CCCGTAT42700.049.0945248
GCTCCCC3600.048.6078244
Produced by FastQC (version 0.11.5)