FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8753153
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2048142.3398882665480656Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG995181.13693888362285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG18450.039.45521545
CCCCGTA22350.039.3039447
AGAGCAC66800.038.82366615
GAGCACA62450.038.49986616
GGTCCCC21750.035.72162244
AAAGGGG379750.033.556267
CGCCGTA586400.033.2729147
CCGTATC586550.032.90118849
TCGCCGT588500.032.8925246
AAGGGGG389950.032.812968
TCCCCGT24750.032.66455546
GCCGTAT605450.032.041448
CGTATCA608450.031.963450
GGTCGCC619350.031.92638444
CGATCGG10000.031.902431
GTCGCCG614150.031.81490345
GTATCAT589450.031.68154151
TGGTCGC628500.031.3893843
GGTGGTC655500.031.03043741
GTGGTCG640600.030.9489242
Produced by FastQC (version 0.11.5)