FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11652158
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3500443.0041130578558928Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1458141.2513905149586884No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC123810.10625499585570329Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA29500.042.70892347
GTCCCCG27250.042.25378845
CGATCGG14050.039.1745341
AGAGCAC82350.038.33471315
GAGCACA77150.038.2860816
GGTCCCC31650.037.26409544
CGCCGTA906850.036.8789747
CCGTATC892400.036.7703749
TCGCCGT909400.036.4906246
TCCCCGT32650.035.6941746
GCCGTAT934200.035.5258148
CGTATCA935650.035.35075850
GGTCGCC972750.035.30849544
GTCGCCG966850.035.26710545
TGGTCGC979500.034.8793843
GTATCAT908700.034.85493551
GTGGTCG1012100.033.966442
GGTGGTC1036900.033.96389841
GTGACTG43800.033.776831
CATTAAA989850.033.49699855
Produced by FastQC (version 0.11.5)