FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5184483
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC915961.7667335392940817Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG558791.077812387464671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC34100.037.77007715
GTCCCCG9500.037.57559245
GAGCACA32650.037.40968316
CCCCGTA10800.034.34911347
GGTCCCC10650.034.17535444
CGCCGTA256750.033.2865447
AAAGGGG172200.033.06806667
TCGCCGT257550.032.87029346
CCGTATC258450.032.6345949
GGTCGCC272300.032.0536944
AAGGGGG178150.031.88535768
GTCGCCG270950.031.83882545
GCCGTAT267500.031.66133548
CGTATCA270900.031.36670750
CTCGGTG274850.031.09418538
GCACACG14200.031.05416318
TGGTCGC280400.031.00323743
GGTGGTC289300.030.94405241
GTGGTCG283950.030.78788242
CGATCGG4350.030.6230811
Produced by FastQC (version 0.11.5)