FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35154038
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12493583.553953033788039Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4329191.2314915287967771No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC428000.12174988261661433Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG107800.046.81397245
CCCCGTA127250.045.84669547
GGTCCCC125700.042.26344344
AGAGCAC313400.041.18579515
GAGCACA300800.040.4887916
TCCCCGT131650.040.1408346
TGGTCCC137800.039.41613443
GTGGTCC135000.038.75599742
CGATCGG43450.038.40581
GCACAAG201100.038.373218
GTGACTG138300.036.0206451
CGCCGTA3386100.035.09617647
CCCGTAT115500.035.088148
CCGTATC3346950.034.93817549
CGCCCTA130850.034.7696147
TCGCCGT3398600.034.67670446
AAAGGGG2332750.034.47873767
GTATCAT3332250.034.0975151
CGTATCA3459350.034.08903550
GGTCGCC3579000.033.99061244
Produced by FastQC (version 0.11.5)