FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18328921
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3031211.6537852937442417Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2172711.1853998388666742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG30550.036.77269445
CGCCGTA814100.036.01169647
TCGCCGT819100.035.54405646
CCGTATC817000.035.23742749
GCCGTAT839900.034.57233448
CCCCGTA34450.034.33683847
GGTCGCC880300.034.31723844
GTCGCCG874700.034.22886745
GGTCCCC35600.033.5224744
CGTATCA866350.033.50461650
TGGTCGC906150.033.23425343
GTGGTCG931200.032.61440742
GGTGGTC958350.032.5486241
CTCGGTG917250.032.385438
GTATCAT853750.032.34351
TCTCGGT936550.031.942437
ATCTCGG948000.031.6411636
CGGTGGT999300.031.2603540
TCGGTGG985300.030.9621739
AGAGCAC99850.030.95043615
Produced by FastQC (version 0.11.5)