FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459848
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1998942.679598833649157Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG911401.2217407110707885No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC82130.11009607702462569Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG16650.042.6686545
CCCCGTA18400.041.46403547
GGTCCCC19500.038.4066344
AGAGCAC48300.036.66689315
GAGCACA46350.035.48795716
CGCCGTA539800.035.25650447
TCCCCGT20350.035.0829246
TCGCCGT540900.034.8222446
CCGTATC534700.034.5457849
GGTCGCC575750.033.86306844
GTCGCCG572600.033.7437645
GCCGTAT555750.033.709648
CGTATCA558550.033.28909350
TGGTCGC584800.033.20160343
GTATCAT543300.032.851951
GTGGTCG601850.032.44085342
GGTGGTC617900.032.37413441
CTCGGTG596150.032.01157438
TGGTCCC23350.031.92439843
TCTCGGT603800.031.65236337
Produced by FastQC (version 0.11.5)