FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5828004
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1924903.3028460515812963Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG688131.1807301436306494No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC58860.1009951262902359Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG16700.046.94293645
CCCCGTA18350.046.15571247
AGAGCAC53500.044.09237315
GAGCACA52150.043.4187716
GTGACTG30200.043.296511
GGTCCCC18500.041.6188144
TCCCCGT19250.040.72487346
TGACTGG33800.039.587852
GCACAAG32900.039.1446118
GACTGGA36150.037.8760223
GTGGTCC20500.037.2166842
TGGTCCC21350.036.71851743
CGCCCTA21750.036.36598647
CGATCGG6750.036.353421
TAACTGA48850.035.3506662
AAAGGGG347350.035.2646467
AAGGGGG352300.034.91906468
GTCGCCC22100.034.68091645
ATAACTG51400.034.441281
AAGAGCA85550.033.50589814
Produced by FastQC (version 0.11.5)