FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473043
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC47441110.606001328402163Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG793201.7732894586526444No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC166560.37236395894249175Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC100780.22530523404313352Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC73440.1641835323291102Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC69520.15541992330500734Illumina Single End PCR Primer 1 (100% over 49bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC68600.15336315792179955Illumina Single End PCR Primer 1 (98% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC55880.12492614088440465Illumina Single End PCR Primer 1 (98% over 50bp)
CGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC54040.12081261011798904Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC53080.11866642015290262Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC51440.11500001229588Illumina Single End PCR Primer 1 (98% over 50bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC49650.11099826225681264Illumina Single End PCR Primer 1 (98% over 50bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG47380.10592341723520207Illumina Single End PCR Primer 1 (97% over 48bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCCCC45350.10138511970486312Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG15000.053.7554971
CCCCGTA35800.053.66887347
GCACAAG47250.052.29209518
AGAGCAC78600.051.87590415
GAGCACA74000.051.7390916
GTGACTG30250.051.2251471
TCCCCGT35300.051.1573646
GTCCCCG34300.051.0157445
TGGTCCC35450.050.3491343
AGCACAA50050.049.57699217
AGATAGA96950.049.5744447
GGTCCCC35800.049.17202844
GTGGTCC35900.049.1325442
CCCGTAT28250.048.3152448
TGACTGG33200.047.8977622
AAGAGCA106750.047.2780614
GATCGAG23000.046.7193878
GATAGAT97800.046.668668
CCCGTAA13650.045.6378748
CTCCCCG3250.045.22662745
Produced by FastQC (version 0.11.5)