FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7248563
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1060921.4636280322044521Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG867121.1962646941193724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA11700.034.39871247
GTCCCCG10800.034.3488645
GAGCACA43200.034.18731316
AGAGCAC47000.033.8069715
CGCCGTA295750.033.32254447
TCGCCGT296800.032.83867346
CCGTATC299600.032.3107249
GGTCGCC314500.032.14823544
GTCGCCG314050.031.71511845
GCCGTAT309450.031.58717248
AAAGGGG206700.031.42597267
CGTATCA314950.030.9575650
TGGTCGC325750.030.84480343
GTGGTCG330950.030.59238242
GGTGGTC341900.030.21673441
CTCGGTG325500.030.20125638
TCTCGGT333350.029.7105237
GTATCAT314750.029.62072851
ATCTCGG336750.029.50346836
AAGGGGG219700.029.42327968
Produced by FastQC (version 0.11.5)