FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9701713
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1617961.6677054866496257Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1183421.21980520347283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA438800.035.2456147
TCGCCGT443000.034.69796846
CCGTATC435350.034.43195349
GGTCGCC472200.033.8638344
CCCCGTA18500.033.86327447
GTCGCCG467400.033.69494245
GCCGTAT454300.033.3963148
GTCCCCG17200.033.37000745
CGTATCA456050.033.15270250
TGGTCGC487450.032.624943
GTGGTCG491450.032.45805442
GGTGGTC506250.032.15189441
GTATCAT452600.032.05991751
AGAGCAC60700.032.05780415
GAGCACA56250.031.91734916
CTCGGTG489400.031.86461838
TCTCGGT501050.031.24214637
ATCTCGG505750.031.1038836
CGGTGGT527750.030.83542840
TCGGTGG523800.030.52025239
Produced by FastQC (version 0.11.5)