FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5568290
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1045781.878099021423094Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615001.1044683376763782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG12000.041.12246345
CCCCGTA14150.040.31589547
GGTCCCC14400.037.91432644
CGCCGTA302100.034.0945347
TCCCCGT14600.033.56017746
TCGCCGT303850.033.49532746
TGGTCCC17150.033.4676243
AGAGCAC35250.033.35964215
CGATCGG5700.033.2065431
CCGTATC305900.033.16758749
GCCGTAT313100.032.71785448
GAGCACA33550.032.64986416
GGTCGCC323050.032.500744
GTCGCCG319700.032.4033845
AAAGGGG231900.031.93535667
CGTATCA320650.031.92507750
TGGTCGC328100.031.76607743
GGTGGTC343700.031.56656541
GTGGTCG333700.031.400842
GTGGTCC17500.031.39834842
Produced by FastQC (version 0.11.5)