FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799385
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC44826411.798330519281409Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG654071.7215154557908716No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC205570.5410612507024164Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC99320.2614107283152405Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC94450.24859286437147066Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC91800.2416180513425199Illumina Single End PCR Primer 1 (100% over 49bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC73300.19292596038569398Illumina Single End PCR Primer 1 (98% over 50bp)
CGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC60450.1591046972075744Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC60390.15894677691257927Illumina Single End PCR Primer 1 (98% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC58230.15326164629275527Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC56140.14776075601709224Illumina Single End PCR Primer 1 (98% over 50bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC53800.1416018645122829Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCCCC46290.12183550758872816Illumina Single End PCR Primer 1 (98% over 50bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG45980.12101958606458678Illumina Single End PCR Primer 1 (97% over 48bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGT44850.11804542050884552Illumina Single End PCR Primer 1 (96% over 32bp)
GGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTG39780.10470115558175862Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG13950.059.076141
GCACAAG42400.053.64763318
GAGCACA67000.053.27560416
GTGACTG27750.053.076811
CCCCGTA37750.052.19360747
GTCCCCG33200.052.17729645
AGAGCAC71750.052.09388415
AGCACAA44700.051.67011617
TGGTCCC37050.051.4781343
GGTCCCC36850.051.3776544
GTGGTCC36350.050.92973742
TGACTGG30050.050.0118982
TCCCCGT36450.049.06245446
CCCCCTA11250.047.9064647
CGCGGAA6100.047.7162971
CCCGTAT28450.046.86715748
CCGTAAC26000.046.7070149
GACTAGT21800.046.5606312
CCCGTAA15500.046.28582848
AAGAGCA95550.046.00407414
Produced by FastQC (version 0.11.5)