FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307745
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152011.1623825745844947No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC136751.0456931588344824Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA31250.046.6991847
TCGCCGT31350.046.2135646
CCGTATC31650.044.89267749
GGTCGCC35750.044.83281344
GTCGCCG35700.044.5035145
GCCGTAT32650.044.4823948
TGGTCGC36550.042.89570643
CGTATCA35250.041.00285750
GTGGTCG39650.040.6893742
CTCGGTG39200.040.44225338
GGTGGTC41100.039.849941
TCTCGGT39850.039.60695337
ATCTCGG40250.038.86406736
GATCTCG40050.038.2717435
CGGTGGT44900.036.9449640
GTATCAT36650.036.6674351
AGATCTC41900.036.66545534
TCGGTGG44700.036.4839339
TAGATCT43800.035.55433333
GTAGATC47400.034.0352732
Produced by FastQC (version 0.11.5)