FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n02_cf-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n02_cf-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977161
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1118965.659427836175203Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307481.555159139796911No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC42740.21616853660374646Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC22400.11329375807028359Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG8750.052.39354345
CCCCGTA9000.050.54932847
CGATCGG4550.047.768581
GGTCCCC9400.047.2814144
TCCCCGT9450.045.9202746
TGGTCCC9750.045.22748643
GTGACTG8950.045.043991
GAGCACA19550.043.14264716
AGAGCAC20350.042.99659315
CCCGTAT7250.042.47752448
GTGGTCC10400.041.72773742
TGACTGG9400.040.9969832
GACCGAC2100.040.00215510
CGCCGTA270850.039.17553347
CCGTATC265900.039.03618249
TCGCCGT272400.038.68282746
GCACAAG12150.038.5980218
GACTGGA10450.038.5451433
CGTATCA273650.038.16083550
GCCGTAT275800.038.15519348
Produced by FastQC (version 0.11.5)