FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25791946
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA4927361.9104258360342412TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3835851.4872278346116266No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2175440.8434571009104935No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1087980.42182935711791586TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG551830.2139543871563627TruSeq Adapter, Index 6 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG473630.1836348447689833TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG412550.15995303340042663TruSeq Adapter, Index 12 (100% over 49bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT305620.11849435478811873TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG297250.11524915568604246TruSeq Adapter, Index 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1386500.045.9396370
GTGACTG152900.044.243261
TGACTGG165100.041.35132
AGGGGGG392550.040.073521
GTCTGCT188900.039.54298456
GCCGTCG186550.038.5975350
TCACAGA1072450.038.37281831
GTCACAG1119550.038.1209230
CCGTCGT197700.037.9788651
GCCGGCG106800.036.67577450
CACAGAT1107450.036.2712432
CGTCGTC195950.036.0491952
GACTGGA191650.035.9136353
TCGTCTG184000.035.89919354
AAAAAAG1877450.035.78388669
GGAGTTC175700.034.97067
GTCGTCT183900.034.94840653
GCTTGAA1608850.034.65981760
CTGCTTG1569450.034.58534658
ACTGGAG199500.034.4129264
Produced by FastQC (version 0.11.5)