FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300870
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG797583.4664279163968414TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107890.4689095863738499No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57080.24808007405894292No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT34010.14781365309643743TruSeq Adapter, Index 9 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC28360.12325772425213072TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG118450.046.8649970
AGGGGGG11700.044.5768281
ACACTCT28850.042.707641
CTACACG32050.040.61812612
CCTACAC32150.040.49178311
TCCCTAC32350.040.1332749
ACGACGC32150.040.0563916
ACACGAC32550.039.99418614
CCCTACA32800.039.68935410
CGACGCT32500.039.30198317
AAAAAAG148000.038.43015369
GACGCTC32850.038.0334818
CACGACG34150.038.01790215
CACTCTT33700.037.4912072
TACACGA34550.037.3751813
TTCCCTA36800.035.6613548
ACGCTCT32450.035.5903719
CTTTCCC37950.033.9366766
CGCTCTT31750.033.72958420
ACTCTTT41050.031.0328393
Produced by FastQC (version 0.11.5)