FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805960
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2735255.691370714695919TruSeq Adapter, Index 8 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1138192.368288541727355No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG979572.0382400186435174TruSeq Adapter, Index 8 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG623841.2980549151470258No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATG529221.1011743751508545TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG403830.8402691657858159TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG296420.6167758366694688TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTG253550.5275740954980899TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATG242960.5055389557965526TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATT239420.49817310173201607TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTATG231520.48173517881963224TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG184990.3849178936154275TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTG176400.3670442533853798TruSeq Adapter, Index 8 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTG152090.31646122730942416TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATT122350.25457973016837426TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGATG105430.21937344463957253TruSeq Adapter, Index 8 (97% over 45bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTC104820.21810418730076822Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG91720.19084636576251154TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTG91490.19036779332329026TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTG83120.1729519180351064TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTTTG75730.15757517748795244TruSeq Adapter, Index 8 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATT74810.15566088773106726TruSeq Adapter, Index 8 (97% over 45bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66890.13918134982396857No Hit
CACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACG66820.13903569734246643Illumina Single End PCR Primer 1 (100% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCAGATCGGAAGAGCACACGT65490.13626830019392588Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATT59750.12432479671075083TruSeq Adapter, Index 8 (97% over 48bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC59370.1235341118111678TruSeq Adapter, Index 14 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACAC58470.12166143704899751Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC53960.11227725574078852TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATT51750.10767879882479253TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTT51650.10747072385121807TruSeq Adapter, Index 8 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT181500.063.7940031
AAAAAGG850500.063.6379170
GTCTGCT165100.059.33495756
GTCGTCT118900.053.9010853
TCGTCTG127700.053.28282554
AGGGGGG77650.053.280341
CGTCTGC216450.053.2467955
CACTCTT223150.053.2164962
AAAAAAG1068600.053.13217569
GCTTGGG47050.053.03715560
ACTCTTT227950.052.1556933
GCTTGTA85750.051.46728560
CGTCGTC134850.051.13358352
GCGTCTG121350.050.2158654
TGCTTGT80600.049.93540259
GCCGTCG133250.049.8073850
CTGCTTG638200.049.5305258
ACGCTCC11500.048.99595619
TCCGGCT75350.048.9102656
CCGTCGT149200.048.84661551
Produced by FastQC (version 0.11.5)