FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8753153
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1266681.4471128289428963TruSeq Adapter, Index 7 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339030.3873232879626347No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG260990.29816684342202177TruSeq Adapter, Index 7 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181130.2069311481245672No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATG110760.12653726034492943TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTG107430.12273291692719183TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATG97650.11155980022284542TruSeq Adapter, Index 7 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG381050.046.15611370
AGGGGGG31350.041.5351031
CGTCTGC81400.041.2334355
GTCTGCT70600.040.649256
CTGCTTG296250.039.9656258
ACACTCT98300.039.8105471
CTACACG103150.039.52661512
CCCTACA103150.039.49268710
TCCCTAC102950.039.46729
CGACGCT102050.039.4035217
ACGACGC104050.039.1176816
CCTACAC104550.038.99732611
ACACGAC106650.038.59063314
GCCGTCG64550.037.52341550
TGCTTGA302100.037.3726659
GTCGTCT60500.036.7946253
CACGACG111800.036.68776315
GCTTGAA330550.036.5704760
GACGCTC108100.036.38970618
TACACGA112800.036.11410513
Produced by FastQC (version 0.11.5)