FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11652158
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4466493.8331869512926273TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG515230.4421756038666829No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248650.21339394814248142No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT167270.14355280798629746TruSeq Adapter, Index 6 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC164390.141081162819797TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG642600.048.5404170
ACACTCT135250.042.316071
CTACACG148750.040.65559412
CCTACAC149550.040.36773711
CGACGCT147850.040.35830317
CCCTACA150650.040.0960410
ACGACGC149900.039.9936616
AGGGGGG47800.039.911081
TCCCTAC151150.039.893439
ACACGAC152750.039.88881714
AAAAAAG808250.039.75280869
GACGCTC153000.038.20023718
CACGACG159250.038.0189615
TACACGA160850.037.59726313
ACGCTCT149650.035.94497319
TTCCCTA174650.034.606628
CACTCTT171700.034.51072
GCTTGAA1001800.033.8832560
CTGCTTG997300.033.57993758
CGCTCTT145500.033.57866720
Produced by FastQC (version 0.11.5)