FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5184483
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG416840.8040145950907738TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG348910.6729889942738746TruSeq Adapter, Index 5 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156270.3014186756905173No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80090.15448020564442008No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG77730.14992816062855255TruSeq Adapter, Index 5 (97% over 41bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG183500.042.3253770
CGTCTGC36800.039.27789755
CTGCTTG172100.036.64438258
GTCTGCT33600.036.14365456
GCCGTCG31750.036.0509850
TGCTTGA177000.034.2458659
GCTTGAA185900.034.0189360
TCTGCTT194650.033.3530557
GCCGGCT31550.033.278556
CCGGCTT38850.032.88121457
TGCCGTC188900.032.77968249
AAAAAAG246750.032.5108769
CTACACG36450.032.4528312
ATGCCGT191150.032.32121348
CCGTCGT37350.032.1449251
AGGGGGG17150.032.0425951
CGGCGTC16650.031.95433652
CCTACAC37500.031.63687111
GCCGTCT162400.031.23168650
ACACGAC38550.031.0481114
Produced by FastQC (version 0.11.5)