FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35154038
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG15701274.466420045401328TruSeq Adapter, Index 4 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2269930.6457096052521761No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1179700.33558022552060734No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT518590.1475193262293225TruSeq Adapter, Index 4 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC517080.1470897880920536TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG2261900.050.8097770
AGGGGGG191650.044.6543241
ACACTCT542250.044.603811
CTACACG597100.042.3761112
CCTACAC597700.042.3391811
CCCTACA600350.042.0648510
ACACGAC609000.041.92740614
CGACGCT593500.041.81851217
AAAAAAG2836900.041.8027569
TCCCTAC606100.041.6647839
ACGACGC601000.041.65303816
CACGACG641150.039.51953515
TACACGA644250.039.253113
GACGCTC622200.039.02515818
CACTCTT657250.037.834562
ACGCTCT599000.037.55665619
TTCCCTA689100.036.8046578
CTTTCCC693100.036.517916
CGCTCTT561350.036.21647320
GCCGTCG200650.034.22743250
Produced by FastQC (version 0.11.5)