FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18328921
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2352631.2835616455545855TruSeq Adapter, Index 3 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG688300.37552674268168873No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG346770.18919280627593954TruSeq Adapter, Index 3 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308020.16805135446871095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG656100.041.7328670
CGTCTGC121050.037.61594855
CTGCTTG561250.037.08412658
AGGGGGG60900.036.0941961
CTACACG155850.036.06395312
ACACGAC156900.036.02345714
CCTACAC157450.035.71960411
CGACGCT154350.035.6429317
ACGACGC158000.035.13049316
CCCTACA160850.034.98633210
GCCGTCG106050.034.9538150
TCCCTAC162400.034.737769
TGCTTGA581200.034.68484559
GTCTGCT111200.034.55819756
GCTTGAA621650.033.98750760
TCTGCTT643350.033.38049757
CCGTCGT119900.033.04715751
TGCCGTC636400.032.99015449
ATGCCGT649650.032.55993748
AAAAAAG879250.032.35102569
Produced by FastQC (version 0.11.5)