FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459848
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1529462.050256251870011TruSeq Adapter, Index 2 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347770.46618912342449875No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG297760.39915022397239197TruSeq Adapter, Index 2 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186360.2498174225533818No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATG86670.11618199191189954TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATG80280.10761613373355597TruSeq Adapter, Index 2 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG355900.048.43594770
AGGGGGG33000.044.1263581
CGTCTGC77150.041.46450855
GTCTGCT65650.041.3160456
CTGCTTG336550.039.1422658
AAAAAAG463750.038.59033269
GCCGTCG63700.038.41081650
GTCGTCT61250.037.48832753
TGCTTGA340950.037.0355559
TCGTCTG64850.036.7016554
CTACACG72150.036.6207612
CCGTCGT71250.036.4528851
CCTACAC73350.036.16478311
TCTGCTT372700.036.14433357
CGACGCT72700.036.0548917
GCTTGAA365900.035.9927360
CCCTACA73450.035.97260710
CGGCGTC31600.035.6675252
ACGACGC73550.035.63845416
TCCCTAC74900.035.2759749
Produced by FastQC (version 0.11.5)