FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5828004
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2364214.0566375726578086TruSeq Adapter, Index 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323190.554546633804644No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA190030.32606360599615236TruSeq Adapter, Index 14 (97% over 38bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160440.27529150632017413No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC86100.14773497066920338TruSeq Adapter, Index 1 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT73340.1258406823330938TruSeq Adapter, Index 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG347600.049.94302770
AGGGGGG26800.042.705341
AAAAAAG425700.041.8327569
ACACTCT74400.039.281021
CTTGAAA610700.032.49335561
GCTTGAA612850.032.21347460
CTACACG97150.031.37589512
CCCTACA97200.031.35948610
TGCTTGA625900.031.32375559
CCTACAC97700.031.27090511
CGACGCT96650.031.21179417
TGAAAAA652850.031.1395663
ACGACGC98050.031.1241216
TTGAAAA641150.031.12349362
CTGCTTG632350.030.9713158
TCCCTAC98600.030.9486489
ACACGAC100050.030.78125814
GAAAAAA672850.030.4227964
TCTGCTT661250.029.7560857
CACTCTT100600.029.7090052
Produced by FastQC (version 0.11.5)