FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473043
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG46540710.404706594593433TruSeq Adapter, Index 22 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG806251.8024642284905377No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405020.9054686038117675No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATG343780.7685595689556304TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTTTG143020.3197375925069354TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC140260.313567296357312TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGGATG124940.27931768149780806TruSeq Adapter, Index 22 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT109260.24426324540139677TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATT103290.2309166265560157TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGGATG78850.1762782070281909TruSeq Adapter, Index 22 (97% over 37bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTC78220.17486976986360292Illumina Single End PCR Primer 1 (100% over 30bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT73220.1636916971287779TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC70240.15702956577882216TruSeq Adapter, Index 22 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAGCTCGTATG54620.12210926655522873TruSeq Adapter, Index 22 (97% over 37bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACG53890.12047726793594428Illumina Single End PCR Primer 1 (100% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCAGATCGGAAGAGCACACGT53000.11848757098914542Illumina Single End PCR Primer 1 (96% over 29bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52840.11812987266163102No Hit
AAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTT48550.10853908625515114RNA PCR Primer, Index 24 (100% over 36bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG45730.10223465323270982TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT157450.060.4672851
AAAAAGG683250.060.06380570
GTCTGCT98100.052.83621656
AAAAAAG820950.051.32361669
CACTCTT191650.051.0066952
GTCGTCT99150.048.68115653
ACTCTTT204150.048.071093
TCGTCTG105050.047.7446754
AGGGGGG62350.047.324421
CGTCTGC129600.047.09791655
CGTCGTC113050.045.79223352
GGATCGG14400.044.7249151
TCCCTAC221250.044.700289
CCCTACA221950.044.559810
CTTTCCC222000.044.473436
ACGCTCC9350.044.16736619
CCTACAC225050.044.11705811
CTACACG225900.044.01302712
ACACGAC228150.043.76304214
TACACGA226950.043.6551913
Produced by FastQC (version 0.11.5)