FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7248563
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG810221.1177663765907808TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192230.2651973915381573No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93190.12856341318962117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG240200.037.1716670
CGTCTGC44300.036.10738455
CTACACG60450.035.14139612
CCCTACA61100.034.82482510
CGACGCT59900.034.7040617
CCTACAC61500.034.59832411
ACACGAC62150.034.29278614
TCCCTAC61800.034.2600149
CTGCTTG206000.034.13188658
ACGACGC61750.034.0050916
GCCGTCG36500.033.1808750
GTCTGCT39950.032.50316656
TGCTTGA209850.032.337859
GCCGGCG23700.031.4583450
GCTTGAA224850.031.13018260
GACGCTC65050.030.99008218
GCCGGCT36500.030.78085756
TCTGCTT236350.030.68334657
TGCCGTC224450.030.64414249
TACACGA70000.030.34710513
Produced by FastQC (version 0.11.5)