FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9701713
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG635170.6546988145289393TruSeq Adapter, Index 20 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304900.3142743966967483No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG277270.28579489003642966TruSeq Adapter, Index 20 (97% over 41bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136800.14100602646151253No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG135530.13969697928602917TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTATG124340.12816293370047124TruSeq Adapter, Index 20 (97% over 41bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG337650.040.05500470
CGTCTGC68050.039.24385555
CTGCTTG223350.038.8302758
CTACACG96000.038.82496312
CCCTACA96550.038.35006310
CGACGCT95750.038.23069417
ACACGAC98350.038.18194614
CCTACAC97400.038.1231811
ACGACGC97350.037.8909216
TCCCTAC99100.037.326989
GCCGTCG50250.037.19675450
CGGCGTC35450.036.13767652
GCCGGCG37500.036.1226550
GTCTGCT63100.035.88733356
TGCTTGA231200.034.98345659
GACGCTC102350.034.98131618
TGCCGTC231150.034.96470649
CACGACG108300.034.5124315
TACACGA109850.033.9935913
GCTTGAA251850.033.94975360
Produced by FastQC (version 0.11.5)