FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5568290
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG1278852.2966655831503027TruSeq Adapter, Index 13 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285980.5135867564368953No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139600.25070533323515837No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG90450.16243766039484292TruSeq Adapter, Index 4 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG237400.043.9641770
AGGGGGG20650.042.3751641
ACGACGC65200.040.6872616
CCTACAC66200.040.3894911
CCCTACA66300.040.3813510
CGACGCT65550.040.3628717
CTACACG66700.040.13954512
ACACGAC68050.039.96039214
TCCCTAC67200.039.944699
ACACTCT65500.039.1700481
CACGACG70600.038.0709215
GACGCTC69100.037.3279518
TACACGA73000.036.62750613
ACGCTCT67200.035.3102819
AAAAAAG316050.034.2964869
TTCCCTA78700.034.2859738
GCCGTCG24000.034.2739150
CACTCTT80150.033.1877752
CGCTCTT64150.033.17016620
CTTTCCC84050.032.313526
Produced by FastQC (version 0.11.5)