FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799385
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG44051711.594429098393556TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG908812.391992388241781No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG629981.6581104573503345TruSeq Adapter, Index 12 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG483581.2727849375622633No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT144000.3790087079882665TruSeq Adapter, Index 12 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC129790.34160791812359104TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATG119020.31326122517196864TruSeq Adapter, Index 12 (97% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT100380.2642006535268208TruSeq Adapter, Index 12 (100% over 48bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTC83700.22029881151817993Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATT77670.20442782187117126TruSeq Adapter, Index 12 (97% over 48bp)
ACACTCTTTCCCTACACGACGCTCTTCCGCCAGATCGGAAGAGCACACGT67810.17847625339364134Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATG66010.173738644543788TruSeq Adapter, Index 12 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC65670.17284376287214903TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTG61120.16086814050168646TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATG54030.1422072256430975TruSeq Adapter, Index 12 (97% over 41bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53800.1416018645122829No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT53800.1416018645122829TruSeq Adapter, Index 12 (100% over 48bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACG51410.13531137276164432Illumina Single End PCR Primer 1 (100% over 32bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTAT51210.1347849717783273TruSeq Adapter, Index 12 (97% over 48bp)
ACACTCTTTCCCTACACGACGCTCTTCCGACAGATCGGAAGAGCACACGT43650.11488701460894328Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACAC41660.10964932482493876Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT184850.063.3946461
AAAAAGG662050.061.20247370
AGGGGGG61950.056.330091
CACTCTT217600.055.054722
GTCTGCT85350.054.2518256
ACTCTTT226500.053.0768473
AAAAAAG794100.052.58239769
GTCGTCT87500.050.76283653
TCCCTAC241550.050.4481749
CTTTCCC241700.050.36166
CCCTACA242450.050.27533710
CCTACAC243150.050.20256411
CTACACG243700.050.11798512
ACACGAC246100.049.98473714
TCGTCTG91700.049.84814554
ACGACGC243550.049.71842616
CGACGCT242300.049.69983717
TACACGA246150.049.61914413
CACGACG245700.049.61031715
TTCCCTA247500.049.34858
Produced by FastQC (version 0.11.5)