FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307745
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG210401.6088763482177335TruSeq Adapter, Index 11 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36010.2753594928674933No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16910.12930655441236633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC10300.043.827659
ACACGAC10400.043.06974814
CGACGCT10500.041.99317
ACGACGC10900.040.7730216
CTACACG11200.040.30578212
CCTACAC11150.040.1726811
CCCTACA11450.039.1201210
CACGACG12050.037.75304415
GACGCTC11600.037.71211618
AAAAAGG41900.036.50168670
TACACGA12650.035.68575313
ACGCTCT11150.035.46765519
CTGCTTG46250.035.18736358
GCCGTCT47200.034.0407650
TGCCGTC49900.034.02277449
TGCTTGA48550.033.8087559
AGGGGGG4350.033.7965161
GCTTGAA49600.033.5176960
ACACTCT12600.033.05891
ATGCCGT51050.033.05316548
Produced by FastQC (version 0.11.5)