FastQCFastQC Report
Fri 16 Aug 2019
HNHLLAFXY_n01_cf-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHLLAFXY_n01_cf-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977161
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG553902.8014916337111644TruSeq Adapter, Index 10 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180370.9122676403186184No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG164740.8332148975222555TruSeq Adapter, Index 10 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98850.4999592850556934No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG66980.3387685676583748TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATG64520.32632648529887043TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATG59450.30068365702135535TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG56030.28338612788741024TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG50030.2530395855471557TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTG43600.22051820767251631TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATT41860.21171771039384246TruSeq Adapter, Index 10 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTG24920.1260393058531905TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG22940.1160249468809065TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGATG20040.10135745141645015TruSeq Adapter, Index 10 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG162100.057.8642870
GTCTGCT32100.053.63964556
AGGGGGG14500.050.9338151
CGTCTGC39400.050.45322855
ACACTCT34850.049.916641
CTGCTTG123350.049.7073758
GTCGTCT25450.047.85994753
TCGTCTG26100.047.73844554
GATGCCG25600.047.72106647
TCTGCTT134650.047.14729357
GCCGTCG26600.046.8470350
AAAAAAG210200.046.83772769
TGCTTGA121500.046.4893159
CCTACAC39550.045.83741411
CTACACG39800.045.72535712
GGATGCC28250.045.5988746
CCCTACA39950.045.4660710
TCCCTAC40200.045.356299
GCTTGAA130500.045.0799160
CCGTCGT30750.044.9640851
Produced by FastQC (version 0.11.5)