FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_sv115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_sv115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29755527
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC893350.300229937113868TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA128100.040.016091
ATCGGAA134250.038.7020532
AATCTCG110950.038.46837239
CGTCTGA134700.038.20158416
ACGTCTG134850.038.1262315
ACACGTC135450.038.02289613
TCGGAAG141600.036.6932753
CGGAAGA147400.035.340984
CTCGTAT122400.034.83299642
TCGTATG122600.034.83140643
TCTCGTA122650.034.780441
CACGTCT148900.034.64864714
CGTATGC124300.034.37290244
GCACACG152150.033.98207511
ATCTCGT131350.032.59683640
CACACGT162450.031.89720512
GTATGCC148050.028.9956645
GTCACAC187800.027.27958529
GAACTCC193700.026.71640621
GATCTCG26050.026.25606338
Produced by FastQC (version 0.11.2)