FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_sv114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_sv114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39254543
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1642560.4184381919820083TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA191050.040.42844841
TCGTATG192900.040.27414343
CTCGTAT192500.040.2291142
CGTATGC194500.040.02376644
CGTCTGA203000.038.9481716
GATCGGA206200.038.722521
ATCTCGT201700.038.38321340
ACACGTC207450.038.2423613
ACGTCTG207500.038.1902615
ATCGGAA213350.037.9269752
CATCTCG205750.037.52916339
TCGGAAG223850.036.0878263
CGGAAGA224850.035.987244
CACGTCT221950.035.82522214
GTATGCC224100.034.89812545
GCACACG234300.034.22421311
CACACGT238950.033.37985612
CTTAGGC249900.031.23277333
TTAGGCA265250.029.5101634
ACTTAGG267150.029.36821632
Produced by FastQC (version 0.11.2)