FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_sv113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_sv113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23201318
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1537290.6625873581837032TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA173650.041.2150641
CGATCTC172700.041.2075438
CACGATC172750.041.1434336
TCGTATG175600.041.0519843
CTCGTAT174950.041.0244642
GATCGGA182200.040.973651
ACGATCT174000.040.92553337
GATCTCG174550.040.92547639
TCACGAT176050.040.5894735
ATCGGAA185550.040.588762
CGTATGC177550.040.58835644
CGTCTGA182100.040.5518516
ACACGTC184500.040.2308813
ATCACGA179500.039.94721634
ACGTCTG185300.039.90003215
ATCTCGT179600.039.83736840
CATCACG181200.039.60951633
TCGGAAG192600.039.0681533
CGGAAGA193900.038.7248234
GTATGCC189800.038.04016545
Produced by FastQC (version 0.11.2)