FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_sv112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_sv112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25253618
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC4162161.6481440401925773TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA460750.043.4760131
ATCGGAA467650.043.248772
CGTCTGA466950.042.97632616
TCTCGTA458250.042.94602241
AATCTCG457950.042.9051239
CTCGTAT458950.042.87553442
ACACGTC469800.042.86785513
TCGTATG461850.042.84040543
ACGTCTG469150.042.8038315
TCGGAAG474150.042.631993
CGTATGC464350.042.60482444
CGGAAGA477750.042.2965284
ATCTCGT466050.042.2611440
CACGTCT479850.041.91409314
GTATGCC479900.041.25269745
GCACACG490150.041.16102611
CACACGT491100.041.07248712
GTAATCT489300.040.11483837
TGTAATC504300.038.89911736
AGTCACC516600.038.6576328