FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_sv111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_sv111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32606735
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC4480911.3742283611039252TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA484500.043.3401341
TCGTATG485550.043.3298143
CTCGTAT484850.043.30864342
GATCGGA492200.043.263351
CGTATGC487800.043.20361744
CGTCTGA493650.043.12190216
ATCGGAA500000.042.8626822
ACACGTC499050.042.7987213
ACGTCTG498850.042.7314315
ATCTCGT493350.042.58117340
CATCTCG493400.042.43080539
TCGGAAG510300.041.997473
CACGTCT510800.041.81472814
CGGAAGA514550.041.694194
GTATGCC513200.041.1662245
GCACACG521900.040.9763111
CACACGT522750.040.88417412
GATCATC527050.039.7735536
GAACTCC558150.038.13854621
ACCAGAT556050.038.04748532
Produced by FastQC (version 0.11.2)