FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_bgm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_bgm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41812044
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28200.026.00944543
CTCGTAT28550.025.8481442
TCTCGTA29550.025.43030541
CGTATGC30600.024.18998144
TATCTCG34850.021.17560239
CGGTCCG28000.018.64220436
ATCTCGT41700.018.23666240
CGTCTGA43900.017.22078516
GTCCGGA47350.016.53573238
ACACGTC47050.016.2112213
ACGTCTG51350.014.98529915
GGTCCGG55450.014.20143537
TCCGGTC40200.014.16000234
TCCGGAA41800.013.94081739
CCGGTCC45750.012.88484135
AGGCCGC50400.012.5065161
CACGTCT65650.011.89243614
CGGGCAC47650.011.8047821
CGGACGG15800.011.24948541
CCGGAAG52750.011.21754940
Produced by FastQC (version 0.11.2)