FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_bgm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_bgm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43626195
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC22765375.218279980640072TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2474950.044.1452261
ATCGGAA2497100.044.0951962
TCGGAAG2500000.043.8594743
CGGAAGA2500800.043.7623564
GTATGCC2440000.043.53405845
ACACGTC2509250.043.19787213
CGTCTGA2502850.043.12150216
ACGTCTG2510600.043.02079415
CACGTCT2524250.042.866314
GCACACG2537700.042.808811
CACACGT2537200.042.7812212
CGATCAG2482100.042.61742833
ACGATCA2497400.042.583132
GTCACGA2499500.042.57598529
GATCTCG2478200.042.52527239
CACGATC2502050.042.5138531
CTCGTAT2485850.042.4893442
TCTCGTA2483800.042.47735641
TCGTATG2500150.042.47044443
TCACGAT2504400.042.47031830