FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_bgm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_bgm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46924609
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC42598239.078014906847704TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4501500.044.779011
ATCGGAA4537000.044.715972
TCGGAAG4544950.044.600173
CGGAAGA4571900.044.3480954
ACACGTC4571250.044.27847313
CGTCTGA4563100.044.26972216
ACGTCTG4569000.044.23215
GCACACG4591050.044.13161511
CACGTCT4582950.044.12251314
CGCCAAT4519350.044.05138433
CACACGT4596600.044.04775212
GTCACGC4549000.044.000729
ACGCCAA4538150.043.9878132
TATCTCG4544350.043.9532439
TCACGCC4547600.043.92339730
AGTCACG4563050.043.912628
GTATGCC4564250.043.88793645
CACGCCA4551450.043.8821531
CTCGTAT4557450.043.8723242
TCTCGTA4559550.043.8688441
Produced by FastQC (version 0.11.2)