FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_bgm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_bgm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48854628
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC562788811.519661965290167TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC6026700.044.3786113
CGTCTGA6019500.044.3497116
ACGTCTG6025250.044.324115
CACGTCT6041450.044.22574614
TCGGAAG6065850.044.1747473
ATCGGAA6073050.044.1657752
GCACACG6063700.044.15727211
CACACGT6059950.044.14550412
CGGAAGA6072350.044.145484
GATCGGA6022700.044.1093751
GTATGCC6001450.044.05098745
GATCTCG5953850.044.03428639
TCTCGTA5970500.043.99835641
CTCGTAT5980700.043.9834242
TCGTATG6001500.043.97633743
CGTATGC6010050.043.9654544
ATCTCGT5976450.043.91449440
AGTGATC6005100.043.58868436
GAACTCC6106050.043.56576521
GTGATCT6015550.043.4837537
Produced by FastQC (version 0.11.2)