FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_bgm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_bgm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43287086
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC850780419.65436989683251TruSeq Adapter, Index 4 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCC664580.15352846805164938TruSeq Adapter, Index 4 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9020900.044.9242321
ATCGGAA9117850.044.8759082
TCGGAAG9117250.044.824823
CGGAAGA9172600.044.5672344
ACACGTC9176200.044.3521713
GCACACG9201100.044.31128311
CACACGT9194650.044.27460512
ACGTCTG9180950.044.2605415
CGTCTGA9182150.044.23760216
CACGTCT9193700.044.22992314
GAAGAGC9288650.044.0388226
GAGCACA9278500.043.9916239
AGAGCAC9299600.043.9413958
AGCACAC9302900.043.8777810
GTATGCC9161100.043.8567745
AATCTCG9065700.043.74990539
GAACTCC9251200.043.74798621
TCTGAAC9276850.043.71626718
GTCTGAA9282300.043.71267717
TCTCGTA9105000.043.6719241
Produced by FastQC (version 0.11.2)