FastQCFastQC Report
Fri 26 Feb 2016
HNHJVBGXX_n01_bgm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNHJVBGXX_n01_bgm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32198192
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC7750422.4070978892230968TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA829400.043.9167671
ATCGGAA837600.043.8528182
CGATGTA826600.043.54572334
TCTCGTA828100.043.50903341
ACCGATG829400.043.5076332
CGTATGC830850.043.5030644
CTCGTAT828650.043.49101342
TCGTATG831250.043.4797643
CACCGAT831400.043.47894731
CGTCTGA839600.043.46988316
ACACGTC841250.043.4613113
CCGATGT829600.043.39618333
TCGGAAG845900.043.3961333
TATCTCG830350.043.32366639
GTCACCG836450.043.2810729
ACGTCTG844850.043.22106615
CGGAAGA851700.043.116264
ATCTCGT837450.043.042440
TCACCGA842500.042.99437330
CACGTCT856750.042.66734714