FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8362236
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG26000.022.7517781
CGCGCAG4200.022.7404941
CGGGCAG8600.021.1368871
GTCGGCG16250.020.86512631
CGGGAAG58500.020.7504631
CGGCCAG5600.020.356411
CGCGAAG10950.019.9770641
TGTCGGC17550.019.7392730
CGCGAAT12100.019.3515471
CGGGCTG4950.019.2949641
CGCGAAA12200.018.6878491
CGGCGGC18350.018.67826533
TCGGCGG19850.018.00933832
CGGCAAA46150.017.9584161
CGCGAGT9150.017.72602563
CGGCAAC18200.017.6055411
GTACGCG9650.017.57017760
CGCCAAT21800.016.3941521
CGCGGAG5850.016.3265081
TACGCGA10800.016.04102561
Produced by FastQC (version 0.11.5)