FastQCFastQC Report
Fri 23 Aug 2019
HNH7FAFXY_n02_vk3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNH7FAFXY_n02_vk3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6542073
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101830.15565402587222735No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG94700.14475533978297095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA68400.036.64467653
GGTCGCC72500.036.43020250
GTCGCCG71800.035.9743651
TCGCCGT70400.035.50059552
CCGTATC67850.035.3326155
GCCGTAT75400.034.73832754
CTCGGTG65050.034.33751344
TGGTCGC76750.034.1767949
TCTCGGT67550.033.1738243
ATCTCGG67450.033.16703842
GTGGTCG79500.033.1315748
CGTATCA74000.032.83804356
GATCTCG66150.031.94806941
GGTGGTC84950.030.62029847
GTATCAT74450.030.09875757
AGATCTC72650.029.74055940
TCGGTGG85050.029.04413445
CGTGTAG98500.028.17425322
AGCGTCG123700.027.927117
GCGTCGT123500.027.92047718
Produced by FastQC (version 0.11.5)